Functional Myotube Formation From Adult Rat Satellite Cells In A Defined Serum-Free System

Keywords

Electrophysiology; In vitro; Muscle; Satellite cells; Serum-free

Abstract

This manuscript describes the development of a culture system whereby mature contracting myotubes were formed from adult rat derived satellite cells. Satellite cells, extracted from the Tibialis Anterior of adult rats, were grown in defined serum-free growth and differentiation media, on a nonbiological substrate, N-1[3-trimethoxysilyl propyl] diethylenetriamine. Myotubes were evaluated morphologically and immunocytochemically, using MyHC specific antibodies, as well as functionally using patch clamp electrophysiology to measure ion channel activity. Results indicated the establishment of the rapid expression of adult myosin isoforms that contrasts to their slow development in embryonic cultures. This culture system has applications in the understanding and treatment of age-related muscle myopathy, muscular dystrophy, and for skeletal muscle engineering by providing a more relevant phenotype for both in vitro and in vivo applications.

Publication Date

7-1-2015

Publication Title

Biotechnology Progress

Volume

31

Issue

4

Number of Pages

997-1003

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/btpr.2063

Socpus ID

84938979623 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84938979623

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