Dynamic Light Scattering Coupled With Gold Nanoparticle Probes As A Powerful Sensing Technique For Chemical And Biological Target Detection

Abstract

Dynamic light scattering (DLS) is an analytical technique used routinely for nanoparticle size measurement. Gold nanoparticles are known for their exceptionally strong light scattering properties. By combining the strong light scattering properties of gold nanoparticle probes with the size measurement capability of DLS, a new chemical and biological sensing technique termed nanoparticle-enabled dynamic light scattering assay (NanoDLSay) was developed. Gold nanoparticles can be surface-modified with various chemical ligands, antibodies or other binding molecules to form gold nanoparticle probes. Binding of specific chemical or biological target analytes with the gold nanoparticle probes leads to nanoparticle cluster formation, and subsequently, an average particle size increase of the assay solution. Such particle size increases can be measured by DLS, and correlated to the quantitative information of target analytes. NanoDLSay is a single-step homogeneous solution assay, easy to perform, of low cost, and has excellent sensitivity and reproducibility. So far, this technique has been applied for quantitative detection and analysis of a wide range of chemical and biological targets, including proteins, DNAs, viruses, carbohydrates, small chemicals, toxic metal ions, food and environmental toxins. In this review, we present both a tutorial explanation on the principle and key factors involved in NanoDLSay technique and a literature update of the field. The analytical performance of NanoDLSay is compared with other sensing techniques. In the Future Outlook section, we discuss further work that needs to be conducted to broaden the applications of NanoDLSay in chemical and biological sensing and quantitative analysis, and to move these applications from laboratories to real world settings.

Publication Date

1-1-2015

Publication Title

ACS Symposium Series

Volume

1215

Number of Pages

157-179

Document Type

Article; Proceedings Paper

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/bk-2015-1215.ch009

Socpus ID

84949678080 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84949678080

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