Dna Nanotechnology For Nucleic Acid Analysis: Multifunctional Molecular Dna Machine For Rna Detection

Abstract

The Nobel prize in chemistry in 2016 was awarded for 'the design and synthesis of molecular machines'. Here we designed and assembled a molecular machine for the detection of specific RNA molecules. An association of several DNA strands, named multifunctional DNA machine for RNA analysis (MDMR1), was designed to (i) unwind RNA with the help of RNA-binding arms, (ii) selectively recognize a targeted RNA fragment, (iii) attract a signal-producing substrate and (iv) amplify the fluorescent signal by catalysis. MDMR1 enabled detection of 16S rRNA at concentrations ∼24 times lower than that by a traditional deoxyribozyme probe.

Publication Date

1-1-2016

Publication Title

Chemical Communications

Volume

52

Issue

99

Number of Pages

14318-14321

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/C6CC06889H

Socpus ID

85002406578 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85002406578

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