Title
Dna Antenna Tile-Associated Deoxyribozyme Sensor With Improved Sensitivity
Keywords
deoxyribozymes; diffusion-limited reactions; DNA crossover tile; mycobacterium tuberculosis; split hybridization probes
Abstract
Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three- to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.
Publication Date
11-3-2016
Publication Title
ChemBioChem
Volume
17
Issue
21
Number of Pages
2038-2041
Document Type
Article
Personal Identifier
scopus
DOI Link
https://doi.org/10.1002/cbic.201600438
Copyright Status
Unknown
Socpus ID
84992491297 (Scopus)
Source API URL
https://api.elsevier.com/content/abstract/scopus_id/84992491297
STARS Citation
Cox, Amanda J.; Bengtson, Hillary N.; Gerasimova, Yulia V.; Rohde, Kyle H.; and Kolpashchikov, Dmitry M., "Dna Antenna Tile-Associated Deoxyribozyme Sensor With Improved Sensitivity" (2016). Scopus Export 2015-2019. 2859.
https://stars.library.ucf.edu/scopus2015/2859