Parainfluenza Virus 5 Upregulates Cd55 Expression To Produce Virions With Enhanced Resistance To Complement-Mediated Neutralization

Keywords

Complement; Parainfluenza virus

Abstract

Many enveloped RNA viruses recruit host cell proteins during assembly as a mechanism to limit antiviral effects of complement. Using viruses which incorporated CD46 alone, CD55 alone or both CD46 and CD55, we addressed the role of these two host cell regulators in limiting complement-mediated neutralization of Parainfluenza virus 5 (PIV5). PIV5 incorporated functional forms of both CD55 and CD46 into virions. PIV5 containing CD55 was highly resistant to complement-mediated neutralization, whereas CD46-containing PIV5 was as sensitive to neutralization as virus lacking both regulators. PIV5 infected cells had increased levels of cell surface CD55, which was further upregulated by exogenous treatment with tumor necrosis factor alpha. PIV5 derived from cells with higher CD55 levels was more resistant to complement-mediated neutralization in vitro than virus from control cells. We propose a role for virus induction of host cell complement inhibitors in defining virus growth and tissue tropism.

Publication Date

10-1-2016

Publication Title

Virology

Volume

497

Number of Pages

305-313

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1016/j.virol.2016.07.030

Socpus ID

84982843279 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84982843279

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