Near-Ir Two-Photon Fluorescent Sensor For K+ Imaging In Live Cells

Keywords

cell imaging; fluorescent sensors; potassium sensors; two-photon absorption; two-photon fluorescence microscopy

Abstract

A new two-photon excited fluorescent K+ sensor is reported. The sensor comprises three moieties, a highly selective K+ chelator as the K+ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (>52-fold) in detecting K+ over other physiological metal cations. Upon binding K+, the sensor switches from nonfluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K+ sensing in living cells.

Publication Date

8-19-2015

Publication Title

ACS Applied Materials and Interfaces

Volume

7

Issue

32

Number of Pages

17565-17568

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1021/acsami.5b04506

Socpus ID

84939839821 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84939839821

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