Title

Pseudomonas Aeruginosa Regulated Intramembrane Proteolysis: Protease Mucp Can Overcome Mutations In The Algo Periplasmic Protease To Restore Alginate Production In Nonmucoid Revertants

Keywords

AlgT/U; Anti-sigma factor; Cystic fibrosis; Mucoid conversion; Prc/AlgO/Tsp; RseP/MucP/YaeL; Sigma factors; Sigma-22; Sigma-E; σ 22; σ E

Abstract

The progression of cystic fibrosis (CF) from an acute to a chronic disease is often associated with the conversion of the opportunistic pathogen Pseudomonas aeruginosa from a nonmucoid form to a mucoid form in the lung. This conversion involves the constitutive synthesis of the exopolysaccharide alginate, whose production is under the control of the AlgT/U sigma factor. This factor is regulated posttranslationally by an extremely unstable process and has been commonly attributed to mutations in the algT (algU) gene. By exploiting this unstable phenotype, we isolated 34 spontaneous nonmucoid variants arising from the mucoid strain PDO300, a PAO1 derivative containing the mucA22 allele commonly found in mucoid CF isolates. Complementation analysis using a minimal tiling path cosmid library revealed that most of these mutants mapped to two protease-encoding genes, algO, also known as prc or PA3257, and mucP. Interestingly, our algO mutations were complemented by both mucP and algO, leading us to delete, clone, and overexpress mucP, algO, mucE, and mucD in both wild-type PAO1 and PDO300 backgrounds to better understand the regulation of this complex regulatory mechanism. Our findings suggest that the regulatory proteases follow two pathways for regulated intramembrane proteolysis (RIP), where both the AlgO/MucP pathway and MucE/AlgW pathway are required in the wild-type strain but where the AlgO/MucP pathway can bypass the MucE/AlgW pathway in mucoid strains with membrane-associated forms of MucA with shortened C termini, such as the MucA22 variant. This work gives us a better understanding of how alginate production is regulated in the clinically important mucoid variants of Pseudomonas aeruginosa.

Publication Date

8-1-2018

Publication Title

Journal of Bacteriology

Volume

200

Issue

16

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1128/JB.00215-18

Socpus ID

85050477965 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85050477965

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