Accurate Pcr Detection Of Influenza A/B And Respiratory Syncytial Viruses By Use Of Cepheid Xpert Flu+Rsv Xpress Assay In Point-Of-Care Settings: Comparison To Prodesse Proflu+

Keywords

CLIA-waived; DNA polymerase; Influenza; Respiratory syncytial virus

Abstract

The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A and B viruses and respiratory syncytial virus (RSV) performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to those of the Prodesse ProFlu+ real-time reverse transcription- PCR (RT-PCR) assay (ProFlu+) for the detection of influenza A and B viruses as well as RSV in a Clinical Laboratory Improvement Amendments (CLIA)-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with results of the ProFlu_ assay in the combined CW and non-CW settings with positive percent agreements (PPA) (100%, 100%, and 97.1%) and negative percent agreements (NPA) (95.2%, 99.5%, and 99.6%) for influenza A and B viruses and RSV, respectively. In conclusion, this multicenter study using the Cepheid Xpert Flu+RSV Xpress Assay demonstrated high sensitivities and specificities for influenza A and B viruses and RSV in ∼60 min for use at the point-of-care in the CW setting.

Publication Date

2-1-2018

Publication Title

Journal of Clinical Microbiology

Volume

56

Issue

2

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1128/JCM.01237-17

Socpus ID

85066840935 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85066840935

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