Keywords
Polycyclic aromatic hydrocarbon metabolites
Abstract
Although environmental monitoring of polycyclic aromatic hydrocarbons (PAH) is an essential step to prevent human exposure to contaminated sites, it provides little information on the actual human uptake and subsequent risks. To this end, urine analysis of short-term biomarkers such as PAH metabolites fill an important niche. The general approach follows the sequence of urine hydrolysis, sample clean-up and pre-concentration, chromatographic separation and determination. Whereas chromatographic methods are based on well established laboratory techniques, the development of easy-to-use, cost-effective and large sample throughput techniques is becoming increasingly relevant to investigate adverse PAH effects on large human populations. This thesis compares the room-temperature, 77K and 4.2K fluorescence properties of 1-naphthol, 2-naphthol, 1-hydroxypyrene, 2-hydroxyfluorene, 3-hydroxybenzopyrene and 9-hydroxyphenanthrene. These metabolites are used as model biomarkers to investigate the analytical potential of a simple method of analysis based on Solid-Phase Extraction and Room-Temperature Fluorimetry. Metabolites are directly determined in the eluting solvent (methanol) without the need of previous separation via multidimensional formats. Metabolite recoveries varied between 87 ± 1.51% (9-hydroxyphenanthrene) and 99 ± 1.05% (3-hydroxybenzopyrene). For 10mL of urine samples, limits of detection varied between 0.01ng.mL-1 (3-hydroxybenzopyrene) and 0.6ng.mL-1 (2-hydroxynaphthalene). These figures of merit demonstrate the potential of this approach for screening purposes
Notes
If this is your thesis or dissertation, and want to learn how to access it or for more information about readership statistics, contact us at STARS@ucf.edu
Graduation Date
2007
Semester
Fall
Advisor
Campiglia, Andres
Degree
Master of Science (M.S.)
College
College of Sciences
Department
Chemistry
Degree Program
Industrial Chemistry
Format
application/pdf
Identifier
CFE0001946
URL
http://purl.fcla.edu/fcla/etd/CFE0001946
Language
English
Release Date
December 2007
Length of Campus-only Access
None
Access Status
Masters Thesis (Open Access)
STARS Citation
Vatsavai, Keerthika, "Analytical Evaluation Of The Fluorescence Characteristics Of Metabolites Of Polycyclic Aromatic Hydrocarbons At Room, Liquid Nitrogen And Liquid Helium Temperatures" (2007). Electronic Theses and Dissertations. 3392.
https://stars.library.ucf.edu/etd/3392