Keywords
Diabetes, Plant-made pharmaceuticals, Molecular farming, Chloroplast biotechnology
Abstract
Current treatment for type I diabetes includes delivery of insulin via injection or pump, which is highly invasive and expensive. The production of chloroplast-derived proinsulin should reduce cost and facilitate oral delivery. Therefore, tobacco and lettuce chloroplasts were transformed with the cholera toxin B subunit fused with human proinsulin (A, B, and C peptides) containing three furin cleavage sites (CTB-PFx3). Transplastomic lines were confirmed for site-specific integration of transgene and homoplasmy. Old tobacco leaves accumulated proinsulin up to 47% of total leaf protein (TLP). Old lettuce leaves accumulated proinsulin up to 53% TLP. Accumulation was so stable that up to ~40% proinsulin in TLP was observed even in senescent and dried lettuce leaves, facilitating their processing and storage in the field. Based on the yield of only monomers and dimers of proinsulin (3 mg/g leaf, a significant underestimation), with a 50% loss of protein during the purification process, one acre of tobacco could yield up to 20 million daily doses of insulin per year. Proinsulin from tobacco leaves was purified up to 98% using metal affinity chromatography without any His-tag. Furin protease cleaved insulin peptides in vitro. Oral delivery of unprocessed proinsulin bioencapsulated in plant cells or injectable delivery into mice showed reduction in blood glucose levels similar to processed commercial insulin. C-peptide should aid in longterm treatment of diabetic complications including stimulation of nerve and renal functions. Hyper-expression of functional proinsulin and exceptional stability in dehydrated leaves offer a low cost platform for oral and injectable delivery of cleavable proinsulin.
Notes
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Graduation Date
2010
Advisor
Daniell, Henry
Degree
Master of Science (M.S.)
College
College of Medicine
Department
Burnett School of Biomedical Sciences
Degree Program
Biotechnology
Format
application/pdf
Identifier
CFE0003257
URL
http://purl.fcla.edu/fcla/etd/CFE0003257
Language
English
Release Date
July 2011
Length of Campus-only Access
None
Access Status
Masters Thesis (Open Access)
STARS Citation
Burberry, Diane, "Low Cost Production Of Proinsulin In Tobacco And Lettuce Chloroplasts For Injectable Or Oral Delivery Of Functional Insulin And" (2010). Electronic Theses and Dissertations. 4340.
https://stars.library.ucf.edu/etd/4340