Keywords
AFM, Impedance, AFM-IR, Multifunctional Fingerprinting, Single Cell, MEMS
Abstract
Single cell analyses to detect cellular responses to external stimuli or stresses are challenging due to the lack of spatial resolution, sensitivity of most traditional tools and assays, and the creation and use of complex processes for electrical measurements. Here we present a sensing platform for multi-functional fingerprinting of individual cells. The approach, derived from Atomic Force Microscopy (AFM) capabilities, aims to collect the topographical, nanomechanical, nanochemical and electrical information on the same single cell. The electrical impedance measurements involve a custom-made MEMS probe with two carbon-printed microrods oriented to form a gap of <50 >µm, while the other techniques use conventional AFM microcantilever probes.
The multifunctional fingerprinting approach is evaluated by studying the effect of external stress, caused by UV exposure, on individual NIH3T3 cells immortalized mouse fibroblasts. Our results reveal a clear difference in morphology, impedance, chemical and mechanical properties of the untreated control compared to the UV treated fibroblasts. Overall, the results showcase the promise of a novel microfabricated device and methodology for multifunctional single cell analyses
Completion Date
2024
Semester
Summer
Committee Chair
Tetard, Laurene and Rajaraman, Swaminathan
Degree
Master of Science (M.S.)
College
College of Graduate Studies
Department
College of Graduate Studies
Degree Program
Nanotechnology
Format
application/pdf
Identifier
DP0028895
Language
English
Rights
In copyright
Release Date
2-15-2026
Length of Campus-only Access
1 year
Access Status
Masters Thesis (Campus-only Access)
Campus Location
Orlando (Main) Campus
STARS Citation
Chang, Ji W., "Multifunctional Fingerprinting of Individual Fibroblasts Using MEMS-Based Devices" (2024). Graduate Thesis and Dissertation 2023-2024. 467.
https://stars.library.ucf.edu/etd2023/467
Accessibility Status
Meets minimum standards for ETDs/HUTs
