Laser-excited time-resolved Shpol'skii spectroscopy for the direct analysis of dibenzopyrene isomers in liquid chromatography fractions

    Authors

    S. J. Yu;A. D. Campiglia

    Comments

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    Abbreviated Journal Title

    Appl. Spectrosc.

    Keywords

    dibenzo[a, l]pyrene; dibenzo[a, e]pyrene; dibenzo[a, h]pyrene; dibenzo[a, i]pyrene; dibenzo[e, l]pyrene; high-performance liquid; chromatography; HPLC; Shpol'skii spectroscopy; laser-excited; time-resolved Shpol'skii spectroscopy; POLYCYCLIC AROMATIC-HYDROCARBONS; ENVIRONMENTAL-SAMPLES; FIBEROPTIC; PROBE; WATER SAMPLES; SPECTROMETRY; DIBENZO, L>PYRENE; HPLC; Instruments & Instrumentation; Spectroscopy

    Abstract

    We present a unique method for the unambiguous determination of dibenzo[a,l]pyrene, dibenzo[a,e]pyrene, dibenzo[a,e]pyrene, dibenzo[a,h]pyrene, and dibenzo[e,l]pyrene in high-performance liquid chromatography (HPLC) fractions. Chemical analysis is performed via laser-excited time-resolved Shpol'skii spectroscopy with the aid of a cryogenic fiber-optic probe, pulsed tunable dye laser, spectrograph, and intensified charge-coupled device. Unambiguous identification is accomplished via wavelength time matrix formats, which give simultaneous access to spectral and lifetime information. Prior to spectroscopic analysis, HPLC fractions are pre-treated with liquid-liquid extraction or solid-liquid extraction at the tip of the fiber-optic probe. Solid-liquid extraction gives the best limits of detection, which vary from 40 pg mL(-1) (dibenzo[a,l]pyrene) to 0.2 ng mL(-1) (dibenzo[e,l]pyrene).

    Journal Title

    Applied Spectroscopy

    Volume

    58

    Issue/Number

    12

    Publication Date

    1-1-2004

    Document Type

    Article

    Language

    English

    First Page

    1385

    Last Page

    1393

    WOS Identifier

    WOS:000225704100002

    ISSN

    0003-7028

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