Title

Differentiating Between Burkitt Lymphoma and CD10+Diffuse Large B-Cell Lymphoma The Role of Commonly Used Flow Cytometry Cell Markers and the Application of a Multiparameter Scoring System

Authors

Authors

P. McGowan; N. Nelles; J. Wimmer; D. Williams; J. G. Wen; M. Li; A. Ewton; C. Curry; Y. L. Zu; A. Sheehan;C. C. Chang

Comments

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Abbreviated Journal Title

Am. J. Clin. Pathol.

Keywords

Flow cytometry; Burkitt lymphoma; CD10+diffuse large B-cell lymphoma; IMMUNOPHENOTYPE; EXPRESSION; Pathology

Abstract

The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of PC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD 71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (13 < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD 71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; chi(2)). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL.

Journal Title

American Journal of Clinical Pathology

Volume

137

Issue/Number

4

Publication Date

1-1-2012

Document Type

Article

Language

English

First Page

665

Last Page

670

WOS Identifier

WOS:000301768400019

ISSN

0002-9173

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