Differentiating Between Burkitt Lymphoma and CD10+Diffuse Large B-Cell Lymphoma The Role of Commonly Used Flow Cytometry Cell Markers and the Application of a Multiparameter Scoring System

    Authors

    P. McGowan; N. Nelles; J. Wimmer; D. Williams; J. G. Wen; M. Li; A. Ewton; C. Curry; Y. L. Zu; A. Sheehan;C. C. Chang

    Comments

    Authors: contact us about adding a copy of your work at [email protected]

    Abbreviated Journal Title

    Am. J. Clin. Pathol.

    Keywords

    Flow cytometry; Burkitt lymphoma; CD10+diffuse large B-cell lymphoma; IMMUNOPHENOTYPE; EXPRESSION; Pathology

    Abstract

    The goal of this study was to evaluate routine flow cytometric (FC) immunophenotypic markers in differentiating between Burkitt lymphoma (BL) and CD10+ diffuse large B-cell lymphoma (DLBCL). We performed retrospective analysis of PC data from 55 patients. We evaluated 9 FC parameters: forward and side scatter (FSC and SSC); mean fluorescent intensity (MFI) for CD20, CD10, CD38, CD79b, CD43, and CD71; and the percentage of neoplastic cells positive for CD71 (%CD71). The FSC; MFIs of CD10, CD43, CD79b, and CD 71; and %CD71 cells were significantly different between BL and CD10+ DLBCL (13 < .05; Student t test). A 5-point scoring system (FSC, %CD71, and MFIs of CD43, CD79b, and CD 71) was devised, and 6 (60%) of 10 BLs scored 3 or greater and 1 (10%) of 10 CD10+ DLBCLs scored 3 (P = .04; chi(2)). Our findings indicate that routine FC parameters can aid in differentiating BL from CD10+ DLBCL.

    Journal Title

    American Journal of Clinical Pathology

    Volume

    137

    Issue/Number

    4

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    665

    Last Page

    670

    WOS Identifier

    WOS:000301768400019

    ISSN

    0002-9173

    Share

    COinS
     

    Accessibility Statement

    This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.