Abstract
Nucleic acid aptamers are "short single-stranded DNA- or RNA-based oligonucleotides that can selectively bind to small molecular ligands or protein targets with high affinity and specificity, when folded into their unique three-dimensional structure" (1). Aptamers have shown promising ability for detection in the subnanomolar range of nucleic acid targets with specificity down to single nucleotide variations (2). The selectivity, low limit of detection, and cost-effectiveness make these nucleic acid aptamers optimal bio-sensors. Previous work by our group has been done to optimize the signal of the dapoxyl-binding aptamer (DAP), which is a light-up aptamer. Here, we propose to organize this aptamer into a split-aptamer system and determine the limit of detection and selectivity for a target gene in Mycobacterium tuberculosis.
Thesis Completion
2021
Semester
Spring
Thesis Chair/Advisor
Gerasimova, Yulia
Degree
Bachelor of Science (B.S.)
College
College of Medicine
Department
Burnett School of Biomedical Sciences
Degree Program
Biomedical Sciences
Language
English
Access Status
Campus Access
Length of Campus-only Access
3 years
Release Date
11-1-2024
Recommended Citation
Mordeson, Jack E., "Split Aptamer Sensor For Single Nucleotide Selective Target Detection" (2021). Honors Undergraduate Theses. 1049.
https://stars.library.ucf.edu/honorstheses/1049