Abstract
Actin is a highly abundant protein in most eukaryotic cells. The assembly of actin monomers to double helical filaments is crucial for many cellular functions, including cell movement and cell division. Actin filament assembly in cells occurs in a crowded intracellular environment consisting of various molecules, including cations and organic osmolytes. Recent studies show that cation binding stiffens actin filaments, and a small organic osmolyte trimethylamine-N-oxide (TMAO) modulates filament assembly. However, how cations and TMAO combined affect actin filament mechanics is not understood. We hypothesize that depending on the concentrations of cations and osmolytes, there will be different effects on the stiffness and assembly of actin filaments. In this study, using TIRF we evaluate actin filament mechanics and assembly. Our findings indicate that when TMAO is present alone, it can increase the elongation rate and stiffness of actin filaments, however the inclusion of potassium levels alongside TMAO reduces the persistence length of actin filaments, suggesting a decrease in filament stiffness compared to the influence of TMAO alone. Furthermore, the elongation rate of actin filaments decreases when both TMAO and potassium ions are present. This study will help us better understand how cations and osmolytes together can affect actin filament mechanics in the living cells.
Thesis Completion
2023
Semester
Fall
Thesis Chair/Advisor
Kang, Ellen
Degree
Bachelor of Science (B.S.)
College
College of Sciences
Department
Physics
Language
English
Access Status
Campus Access
Length of Campus-only Access
1 year
Release Date
12-15-2024
Recommended Citation
Kalae, Abdulrazak, "Impact of Osmolytes and Cation on Actin Filament Assembly and Mechanics" (2023). Honors Undergraduate Theses. 1536.
https://stars.library.ucf.edu/honorstheses/1536