Abstract
As we face an expanding elderly population and increased age-related chronic diseases, we must seek solutions that address interconnected problems. Dasatinib plus quercetin (D+Q), a novel combination drug, is a proposed solution to improve the health and longevity of aged individuals with minimal side effects, exceptional efficacy, and maximum convenience. In this experiment, intermittent D+Q treatment was completed on young and old mice. RNA was extracted from perigonadal adipose tissue and cDNA libraries of mRNA and miRNA were made. Real-time quantitative polymerase chain reaction was used to assess relative gene expression for mRNA and miRNA pertaining to cell senescence, inflammation, and insulin resistance. miR-146a was found to have a significant difference (p < 0.05) in relative expression based on the interaction between age and treatment, while other genes showed no difference in relative expression. Furthermore, miR-149 was found to show near significance in interaction (p=0.0552) and had significant difference between old control and old D+Q treated mice. The miR-149 presents an avenue for future research in senescence markers and shows potential as a novel senolytic target.
Thesis Completion
2020
Semester
Spring
Thesis Chair/Advisor
Masternak, Michal M.
Degree
Bachelor of Science (B.S.)
College
College of Medicine
Department
Biomedical Sciences
Degree Program
Biotechnology
Language
English
Access Status
Open Access
Length of Campus-only Access
3 years
Release Date
5-1-2023
Recommended Citation
Hernandez, Catalina, "The Effect of a Senolytic Drug on Messenger RNA and Micro RNA Expression in Perigonadal Adipose Tissue of Young and Old Mice" (2020). Honors Undergraduate Theses. 715.
https://stars.library.ucf.edu/honorstheses/715