Keywords
Gtpase, cross talk, wing veins
Abstract
The Ras superfamily of GTPases are important regulators of morphogenesis involved in control of cytoskeletal dynamics, intracellular trafficking, apical-basal polarity and cell migration. Mis-regulation of GTPase signaling interferes with development and is linked to pathogenesis. Traditionally, GTPase signaling has been depicted as a series of independent linear pathways. However, recently it has become apparent that multiple GTPases can interact to regulate a single cellular process, functioning in poorly understood networks of cross talk between pathways during development. Jim Fristrom (unpublished data) identified a mutation (18-5) that interacts with components of the GTPases Rho1, Rala, and Cdc42 signaling in multiple developmental contexts. Genetic analysis, physical mapping studies, and sequencing of the mutant allele have indicated that the gene was an allele of GEFmeso (CG30115), which encodes guanine nucleotide exchange factor. To show that 18-5 is an allele of GEFmeso, I demonstrated that a GEFmeso transgene could functionally rescue developmental defects associated with the 18-5 mutation. I also investigated cross talk and network variation in signaling interactions between GEFmeso and other GTPase signaling components in the Drosophila wing. My data provide evidence for microenvironment-dependent variation in GTPase signaling networks in specific domains of the wing, and reveal intercellular variation in GTPase signaling within an otherwise uniform epithelium.
Notes
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Graduation Date
2012
Semester
Spring
Advisor
von Kalm, Laurence
Degree
Master of Science (M.S.)
College
College of Sciences
Department
Biology
Degree Program
Biology
Format
application/pdf
Identifier
CFE0004252
URL
http://purl.fcla.edu/fcla/etd/CFE0004252
Language
English
Release Date
May 2013
Length of Campus-only Access
1 year
Access Status
Masters Thesis (Open Access)
Subjects
Dissertations, Academic -- Sciences, Sciences -- Dissertations, Academic
STARS Citation
Iketani, Ashley Megan, "Genetic Interactions Between The Guanine Nucleotide Exchange Factor Gefmeso And Gtpase Signaling Components In The Drosophila Wing Reveal Microenvironment Dependent Variation Within Gtpase Signaling N" (2012). Electronic Theses and Dissertations. 2456.
https://stars.library.ucf.edu/etd/2456